| Frederick Douglass/Greater Rochester International → Chicago O'Hare International ROC–ORD | 2,399 | 180,339 | 81% | 528 | Envoy Air, Republic Airline, United Air Lines Inc., SkyWest Airlines Inc. +1 | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → John F Kennedy International ROC–JFK | 1,594 | 126,121 | 78% | 264 | Endeavor Air Inc., JetBlue Airways, Delta Air Lines Inc. | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Detroit Metro Wayne County ROC–DTW | 1,289 | 87,128 | 85% | 296 | Endeavor Air Inc., SkyWest Airlines Inc., Delta Air Lines Inc. | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Philadelphia International ROC–PHL | 1,272 | 55,809 | 88% | 257 | Piedmont Airlines, Federal Express Corporation, PSA Airlines Inc., Southwest Airlines Co. +3 | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Washington Dulles International ROC–IAD | 1,228 | 58,040 | 86% | 287 | CommuteAir LLC dba CommuteAir, GoJet Airlines LLC d/b/a..., Republic Airline, Mesa Airlines Inc. +1 | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Baltimore/Washington International Thurgood Marshall ROC–BWI | 1,090 | 148,609 | 89% | 277 | Southwest Airlines Co., Endeavor Air Inc. | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Hartsfield/Jackson Atlanta International ROC–ATL | 1,014 | 140,815 | 84% | 749 | Delta Air Lines Inc., Southwest Airlines Co. | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Newark Liberty International ROC–EWR | 981 | 52,932 | 81% | 246 | Republic Airline, GoJet Airlines LLC d/b/a..., United Air Lines Inc., Federal Express Corporation +1 | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Charlotte/Douglas International ROC–CLT | 980 | 105,522 | 81% | 573 | American Airlines Inc., PSA Airlines Inc., Envoy Air, Southwest Airlines Co. +1 | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Ronald Reagan Washington Ntl ROC–DCA | 973 | 52,257 | 77% | 296 | PSA Airlines Inc., Republic Airline, Envoy Air | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Laguardia ROC–LGA | 916 | 51,822 | 75% | 254 | Endeavor Air Inc., SkyWest Airlines Inc., Southwest Airlines Co., Delta Air Lines Inc. +1 | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → General Edward Lawrence Logan International ROC–BOS | 811 | 33,625 | 67% | 343 | Piedmont Airlines, Republic Airline, Envoy Air, Federal Express Corporation | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Orlando International ROC–MCO | 491 | 69,280 | 84% | 1,033 | Southwest Airlines Co., Spirit Air Lines, JetBlue Airways | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Chicago Midway International ROC–MDW | 488 | 59,969 | 79% | 523 | Southwest Airlines Co. | Jun 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Raleigh-Durham International ROC–RDU | 287 | 32,479 | 74% | 503 | Breeze Aviation Group DBA Breeze, TEM Enterprises dba Avelo Airlines | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Frederick W Smith Intl/Memphis ROC–MEM | 206 | 0 | | 863 | Federal Express Corporation | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Concord ROC–USA | 201 | 26,356 | 78% | 558 | TEM Enterprises dba Avelo Airlines | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Harry Reid International ROC–LAS | 148 | 20,076 | 80% | 2,040 | Southwest Airlines Co. | Jan 2025 – Mar 2026 |
| Frederick Douglass/Greater Rochester International → Nashville International ROC–BNA | 120 | 12,971 | 62% | 680 | TEM Enterprises dba Avelo Airlines, Federal Express Corporation, SkyWest Airlines Inc. | Jan 2025 – Jun 2026 |
| Frederick Douglass/Greater Rochester International → Tampa International ROC–TPA | 120 | 14,007 | 79% | 1,079 | Breeze Aviation Group DBA Breeze, Southwest Airlines Co., JetBlue Airways | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Charleston Afb/Intl ROC–CHS | 110 | 10,326 | 76% | 717 | Breeze Aviation Group DBA Breeze | May 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Lakeland Linder International ROC–LAL | 103 | 15,631 | 83% | 1,071 | TEM Enterprises dba Avelo Airlines | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Myrtle Beach International ROC–MYR | 98 | 9,999 | 72% | 655 | Breeze Aviation Group DBA Breeze, Spirit Air Lines | Apr 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Cincinnati/Northern Kentucky International ROC–CVG | 85 | 0 | | 461 | Kalitta Charters II, ABX Air Inc | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Wilmington International ROC–ILM | 81 | 8,939 | 69% | 611 | TEM Enterprises dba Avelo Airlines | Apr 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Punta Gorda ROC–PGD | 70 | 10,169 | 81% | 1,143 | Allegiant Air | Jan 2025 – May 2026 |
| Frederick Douglass/Greater Rochester International → Fort Lauderdale/Hollywood International ROC–FLL | 53 | 6,817 | 67% | 1,183 | Spirit Air Lines, Allegiant Air | Jan 2025 – May 2026 |
| Frederick Douglass/Greater Rochester International → Sarasota/Bradenton International ROC–SRQ | 41 | 5,601 | 76% | 1,118 | Allegiant Air, JetBlue Airways | Feb 2026 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Southwest Florida International ROC–RSW | 38 | 3,955 | 74% | 1,166 | Breeze Aviation Group DBA Breeze, Southwest Airlines Co., Spirit Air Lines | Feb 2025 – May 2026 |
| Frederick Douglass/Greater Rochester International → Indianapolis International ROC–IND | 28 | 98 | 72% | 505 | Federal Express Corporation, Southwest Airlines Co. | Apr 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Miami International ROC–MIA | 22 | 1,588 | 95% | 1,203 | Republic Airline | Jan 2025 – Apr 2026 |
| Frederick Douglass/Greater Rochester International → Buffalo Niagara International ROC–BUF | 13 | 945 | 67% | 55 | Piedmont Airlines, Southwest Airlines Co., Republic Airline, Delta Air Lines Inc. +2 | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Frederick Douglass/Greater Rochester International ROC–ROC | 12 | 0 | 0% | 0 | SkyWest Airlines Inc. | Jan 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Syracuse Hancock International ROC–SYR | 8 | 410 | 75% | 79 | Endeavor Air Inc., Delta Air Lines Inc., Federal Express Corporation, Envoy Air +4 | Jan 2025 – Jun 2026 |
| Frederick Douglass/Greater Rochester International → Toronto ROC–YYZ international | 5 | 147 | 52% | 106 | Endeavor Air Inc., Chartright Air Inc., Piedmont Airlines, Republic Airline | Feb 2025 – Mar 2026 |
| Frederick Douglass/Greater Rochester International → Atlantic City International ROC–ACY | 4 | 328 | 44% | 299 | Sun Country Airlines d/b/a... | May 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Norfolk International ROC–ORF | 4 | 4 | 50% | 436 | Federal Express Corporation, CFM Inc d/b/a Contour... | Jul 2025 – Jun 2026 |
| Frederick Douglass/Greater Rochester International → Albany International ROC–ALB | 3 | 187 | 97% | 198 | Air Hamburg..., Piedmont Airlines, Southwest Airlines Co. | May 2025 – Mar 2026 |
| Frederick Douglass/Greater Rochester International → Westchester County ROC–HPN | 3 | 10 | 42% | 248 | Tradewind Aviation | May 2025 – Jan 2026 |
| Frederick Douglass/Greater Rochester International → Blue Bell ROC–BBX | 2 | 3 | 19% | 241 | Tradewind Aviation | Jun 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Bradley International ROC–BDL | 2 | 2 | 25% | 267 | Federal Express Corporation, Tradewind Aviation | Jan 2025 – Nov 2025 |
| Frederick Douglass/Greater Rochester International → Birmingham-Shuttlesworth International ROC–BHM | 2 | 138 | 62% | 822 | Delta Air Lines Inc., SkyWest Airlines Inc. | Jul 2026 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Republic ROC–FRG | 2 | 95 | 51% | 274 | Allegiant Air, Tradewind Aviation | Aug 2025 – Sep 2025 |
| Frederick Douglass/Greater Rochester International → Ithaca Tompkins International ROC–ITH | 2 | 77 | 77% | 75 | CommuteAir LLC dba CommuteAir | Jul 2025 – Oct 2025 |
| Frederick Douglass/Greater Rochester International → Laredo International ROC–LRD | 2 | 0 | | 1,624 | USA Jet Airlines Inc. | Oct 2025 – Oct 2025 |
| Frederick Douglass/Greater Rochester International → Pittsburgh International ROC–PIT | 2 | 44 | 31% | 224 | PSA Airlines Inc., SkyWest Airlines Inc. | Feb 2025 – Mar 2026 |
| Frederick Douglass/Greater Rochester International → Portland International Jetport ROC–PWM | 2 | 7 | 44% | 372 | CFM Inc d/b/a Contour... | Jul 2026 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Savannah/Hilton Head International ROC–SAV | 2 | 181 | 55% | 782 | Delta Air Lines Inc., Southwest Airlines Co. | Apr 2026 – Jun 2026 |
| Frederick Douglass/Greater Rochester International → South Bend International ROC–SBN | 2 | 0 | 0% | 453 | Federal Express Corporation, SkyWest Airlines Inc. | Mar 2026 – May 2026 |
| Frederick Douglass/Greater Rochester International → Louisville Muhammad Ali International ROC–SDF | 2 | 0 | 0% | 544 | USA Jet Airlines Inc., Republic Airline | Nov 2025 – Jan 2026 |
| Frederick Douglass/Greater Rochester International → Willow Run ROC–YIP | 2 | 0 | | 304 | USA Jet Airlines Inc. | Jul 2025 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Lehigh Valley International ROC–ABE | 1 | 0 | | 205 | Federal Express Corporation | Jun 2025 – Jun 2026 |
| Frederick Douglass/Greater Rochester International → Augusta Regional AT Bush Field ROC–AGS | 1 | 155 | 99% | 712 | Delta Air Lines Inc. | Jun 2026 – Jun 2026 |
| Frederick Douglass/Greater Rochester International → Watertown International ROC–ART | 1 | 34 | 68% | 103 | Piedmont Airlines | May 2025 – Nov 2025 |
| Frederick Douglass/Greater Rochester International → Birmingham ROC–BHX international | 1 | 2 | 13% | 3,426 | Sparfell Luftfahrt GmbH | Jan 2026 – Jan 2026 |
| Frederick Douglass/Greater Rochester International → Cleveland-Hopkins International ROC–CLE | 1 | 74 | 97% | 245 | Endeavor Air Inc. | Feb 2026 – Feb 2026 |
| Frederick Douglass/Greater Rochester International → Erie Intl/Tom Ridge Field ROC–ERI | 1 | 33 | 66% | 147 | Piedmont Airlines | Jun 2026 – Jun 2026 |
| Frederick Douglass/Greater Rochester International → Francis S Gabreski ROC–FOK | 1 | 0 | 0% | 303 | CFM Inc d/b/a Contour... | Apr 2026 – Apr 2026 |
| Frederick Douglass/Greater Rochester International → Fort Wayne International ROC–FWA | 1 | 9 | 12% | 414 | Envoy Air | Apr 2026 – Apr 2026 |
| Frederick Douglass/Greater Rochester International → Piedmont Triad International ROC–GSO | 1 | 123 | 96% | 499 | American Airlines Inc. | Nov 2025 – Nov 2025 |
| Frederick Douglass/Greater Rochester International → Greenville Spartanburg International ROC–GSP | 1 | 147 | 94% | 618 | Delta Air Lines Inc. | Aug 2025 – Aug 2025 |
| Frederick Douglass/Greater Rochester International → Long Island Mac Arthur ROC–ISP | 1 | 0 | 0% | 285 | Southwest Airlines Co. | May 2026 – May 2026 |
| Frederick Douglass/Greater Rochester International → LA Crosse Regional ROC–LSE | 1 | 33 | 43% | 684 | Envoy Air | Mar 2026 – Mar 2026 |
| Frederick Douglass/Greater Rochester International → Harrisburg International ROC–MDT | 1 | 47 | 94% | 207 | Piedmont Airlines | Mar 2025 – Oct 2025 |
| Frederick Douglass/Greater Rochester International → General Mitchell International ROC–MKE | 1 | 0 | 0% | 518 | Endeavor Air Inc. | Feb 2026 – Feb 2026 |
| Frederick Douglass/Greater Rochester International → Minneapolis-St Paul Intl/Wold-Chamberlain ROC–MSP | 1 | 0 | 0% | 783 | SkyWest Airlines Inc. | Nov 2025 – Nov 2025 |
| Frederick Douglass/Greater Rochester International → Canandaigua ROC–N1Y | 1 | 1 | 12% | 23 | Tradewind Aviation | Aug 2025 – Aug 2025 |
| Frederick Douglass/Greater Rochester International → Brunswick ROC–NHZ | 1 | 4 | 50% | 392 | CFM Inc d/b/a Contour... | Jul 2026 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Newport ROC–NPT | 1 | 2 | 25% | 345 | Tradewind Aviation | Jun 2026 – Jun 2026 |
| Frederick Douglass/Greater Rochester International → Norwood Memorial ROC–OWD | 1 | 8 | 100% | 337 | Tradewind Aviation | Oct 2025 – Oct 2025 |
| Frederick Douglass/Greater Rochester International → Pau ROC–PUF international | 1 | 5 | 36% | 3,747 | VistaJet Limited | Oct 2025 – Oct 2025 |
| Frederick Douglass/Greater Rochester International → Rhode Island TF Green International ROC–PVD | 1 | 2 | 1% | 333 | Southwest Airlines Co. | Mar 2026 – Mar 2026 |
| Frederick Douglass/Greater Rochester International → Phoenix ROC–SCF | 1 | 6 | 75% | 1,955 | Jet Aviation Flight Services, Inc. | Jul 2026 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Eugene F Kranz Toledo Express ROC–TOL | 1 | 0 | 0% | 331 | Sun Country Airlines d/b/a... | Jul 2026 – Jul 2026 |
| Frederick Douglass/Greater Rochester International → Montreal ROC–YUL international | 1 | 39 | 56% | 254 | Air Canada | Mar 2026 – Mar 2026 |